4V5K

Structure of cytotoxic domain of colicin E3 bound to the 70S ribosome

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-06
Detector	ADSC
Spacegroup name	P 21 21 21
Unit cell lengths	212.041, 453.514, 616.103
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.530 - 3.200
R-factor	0.228
Rwork	0.228
R-free	0.27000
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.020
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	44.530
High resolution limit [Å]	3.200
Rmerge	0.110
Number of reflections	960332
<I/σ(I)>	10.95
Completeness [%]	99.4
Redundancy	4.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.18		AS STATED IN STRUCTURE OF THE 70S RIBOSOME COMPLEXED WITH MRNA AND TRNA. (2006) SCIENCE 313: 1935, PH 7.18