4V5J
Structure of the 70S ribosome bound to Release factor 2 and a substrate analog provides insights into catalysis of peptide release
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.421, 452.497, 625.444 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.860 - 3.100 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2J00 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.200 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.160 | 0.720 |
| Number of reflections | 1071522 | |
| <I/σ(I)> | 10.42 | 1.94 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 8.85 | 7.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | SEE SELMER ET AL., SCIENCE 2006, pH 6.5 |
