4V5F
The structure of the ribosome with elongation factor G trapped in the post-translocational state
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | PSI PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 291.839, 270.358, 402.363 |
Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.600 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2J00 2J01 2J02 2J03. |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.700 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.370 | 1.150 |
Number of reflections | 690340 | |
<I/σ(I)> | 6.64 | 1.54 |
Completeness [%] | 96.0 | 89.9 |
Redundancy | 9.2 | 6.91 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP |