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4V5F

The structure of the ribosome with elongation factor G trapped in the post-translocational state

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
DetectorPSI PILATUS 6M
Spacegroup nameP 1 21 1
Unit cell lengths291.839, 270.358, 402.363
Unit cell angles90.00, 91.73, 90.00
Refinement procedure
Resolution50.000 - 3.600
R-factor0.227
Rwork0.227
R-free0.26000
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)PDB ENTRIES 2J00 2J01 2J02 2J03.
RMSD bond length0.008
RMSD bond angle1.200
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER
Refinement softwareCNS (1.2)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0003.700
High resolution limit [Å]3.6003.600
Rmerge0.3701.150
Number of reflections690340
<I/σ(I)>6.641.54
Completeness [%]96.089.9
Redundancy9.26.91
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
16.5100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP

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