4V5E
Insights into translational termination from the structure of RF2 bound to the ribosome
This is a non-PDB format compatible entry.
Replaces: 2JL5Replaces: 2JL6Replaces: 2JL7Replaces: 2JL8Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 211.341, 450.908, 614.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.570 - 3.450 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2J00 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.500 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.210 | 0.900 |
| Number of reflections | 759980 | |
| <I/σ(I)> | 8.19 | 1.74 |
| Completeness [%] | 99.6 | 99.5 |
| Redundancy | 5.46 | 3.63 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.1 | SEE SELMER ET AL., SCIENCE 2006, pH 7.1 |
