4V57
Crystal structure of the bacterial ribosome from Escherichia coli in complex with spectinomycin and neomycin.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2006-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.111 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 208.848, 379.202, 739.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.000 - 3.500 |
| Rwork | 0.264 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2AVY 2AW4 2AW7 2AWB |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 182.000 | 3.850 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.104 | 0.410 |
| Number of reflections | 521699 | |
| <I/σ(I)> | 8.6 | 1.9 |
| Completeness [%] | 72.1 | 13.9 |
| Redundancy | 3.3 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch | 7.5 | 277 | MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | MPD | ||
| 11 | 1 | 2 | PEG 8000 | ||
| 12 | 1 | 2 | MgCl2 | ||
| 13 | 1 | 2 | NH4Cl | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | NH4Cl | ||
| 5 | 1 | 1 | spermine | ||
| 6 | 1 | 1 | spermidine | ||
| 7 | 1 | 1 | TRIS | ||
| 8 | 1 | 1 | EDTA | ||
| 9 | 1 | 1 | H2O |
