4V4Z
70S Thermus thermophilous ribosome functional complex with mRNA and E- and P-site tRNAs at 4.5A.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-06 |
| Detector | MAR CCD 225 mm |
| Wavelength(s) | 0.89888 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 508.650, 508.650, 803.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 300.000 - 4.510 |
| R-factor | 0.3 |
| Rwork | 0.298 |
| R-free | 0.34500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.107 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 300.000 | 4.580 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.108 | |
| Number of reflections | 299163 | |
| <I/σ(I)> | 9 | 1.8 |
| Completeness [%] | 97.5 | 97.8 |
| Redundancy | 5.8 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | MPD, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | H2O | ||
| 3 | 1 | 2 | MPD | ||
| 4 | 1 | 2 | H2O |
