4V4T
Crystal structure of the whole ribosomal complex with a stop codon in the A-site.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.976269 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 517.414, 517.414, 365.385 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 6.760 |
| High resolution limit [Å] | 6.460 | 6.460 |
| Number of reflections | 123391 | |
| <I/σ(I)> | 10.1 | 2.625 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.5 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.7 | 277 | PEG20k, MES buffer, magnesium acetate, potassium chloride, ammonium chloride, pH 6.7, VAPOR DIFFUSION, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG20K | ||
| 10 | 1 | 2 | ammonium chloride | ||
| 2 | 1 | 1 | MES | ||
| 3 | 1 | 1 | magnesium acetate | ||
| 4 | 1 | 1 | potassium chloride | ||
| 5 | 1 | 1 | ammonium chloride | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG20K | ||
| 8 | 1 | 2 | magnesium acetate | ||
| 9 | 1 | 2 | potassium chloride |
