EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
| Method | Blot for 2-2.5s before plunging |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 160 |
| Cs (mm) | 2 |
| Detector | GENERIC TVIPS (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.9 |
| Software | Flex-EM,MODELLER,UCSF Chimera,EMAN |
| Symmetry | D8 |
