4V4H
Crystal structure of the bacterial ribosome from Escherichia coli in complex with the antibiotic kasugamyin at 3.5A resolution.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-12 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 208.848, 379.202, 739.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.000 - 3.460 |
| R-factor | 0.279 |
| Rwork | 0.279 |
| R-free | 0.33100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1PNS 1PNU |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.160 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 3.550 |
| High resolution limit [Å] | 3.460 | 3.460 |
| Rmerge | 0.145 | 0.594 |
| Number of reflections | 693093 | |
| <I/σ(I)> | 7.4 | 2 |
| Completeness [%] | 92.3 | 87.9 |
| Redundancy | 6.2 | 0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | MPD, PEG 8000, MGCL2, NH4CL, SPERMINE, SPERMIDINE, TRIS-HCL, EDTA, PH 7.5, BATCH, TEMPERATURE 277K, pH 7.50 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | MPD | ||
| 11 | 1 | 2 | PEG 8000 | ||
| 12 | 1 | 2 | MgCl2 | ||
| 13 | 1 | 2 | NH4Cl | ||
| 2 | 1 | 1 | PEG 8000 | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | NH4Cl | ||
| 5 | 1 | 1 | spermine | ||
| 6 | 1 | 1 | spermidine | ||
| 7 | 1 | 1 | TRIS-HCL | ||
| 8 | 1 | 1 | EDTA | ||
| 9 | 1 | 1 | H2O |
