4V2Z
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Pomalidomide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-11-26 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.469, 60.144, 88.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.350 - 1.450 |
R-factor | 0.16961 |
Rwork | 0.168 |
R-free | 0.20254 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.021 |
RMSD bond angle | 2.203 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.300 | 1.540 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.040 | 0.450 |
Number of reflections | 54054 | |
<I/σ(I)> | 16.32 | 2.45 |
Completeness [%] | 99.5 | 99.1 |
Redundancy | 3.4 | 3.37 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1 M SODIUM ACETATE PH 4.6, 15 %(W/V) PEG 20000 |