4V27
Structure of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with mannose-alpha-1,3-isofagomine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-29 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | I 4 |
| Unit cell lengths | 108.280, 108.280, 67.620 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.280 - 1.900 |
| R-factor | 0.15245 |
| Rwork | 0.151 |
| R-free | 0.18807 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ad1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.175 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.6.0119) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.400 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.050 | 0.200 |
| Number of reflections | 30894 | |
| <I/σ(I)> | 23.2 | 8.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.1 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 2.8 M SODIUM ACETATE PH 6.4 |
