4V11
Structure of Synaptotagmin-1 with SV2A peptide phosphorylated at Thr84
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-17 |
| Detector | RIGAKU SATURN 994 CCD-DETECTOR |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.049, 41.432, 48.014 |
| Unit cell angles | 90.00, 96.59, 90.00 |
Refinement procedure
| Resolution | 31.279 - 1.950 |
| R-factor | 0.1534 |
| Rwork | 0.151 |
| R-free | 0.19560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tjx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.004 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (PHASER) |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.280 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.010 | 0.030 |
| Number of reflections | 13172 | |
| <I/σ(I)> | 58.93 | 23.39 |
| Completeness [%] | 99.0 | 98.2 |
| Redundancy | 2 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 18 % PEG 3350, 0.05 M CACL2 |
