4V0X

The crystal structure of mouse PP1G in complex with truncated human PPP1R15B (631-684)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-01-17
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 41 21 2
Unit cell lengths	67.540, 67.540, 158.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.337 - 1.850
R-factor	0.1789
Rwork	0.177
R-free	0.22220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	THE CRYSTAL STRUCTURE OF MOUSE PP1G IN COMPLEX WITH TRUNCATED HUMAN PPP1R15B INCLUDING 631-660
RMSD bond length	0.011
RMSD bond angle	1.221
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	CCP4I
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.340	1.890
High resolution limit [Å]	1.850	1.850
Rmerge	0.090	0.980
Number of reflections	32078
<I/σ(I)>	9.7	1.5
Completeness [%]	100.0	100
Redundancy	7.8	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		2.0 M SODIUM MALONATE, PH6.0