4V0W
The crystal structure of mouse PP1G in complex with truncated human PPP1R15B (631-669)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.010, 67.860, 156.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.936 - 1.550 |
| R-factor | 0.1743 |
| Rwork | 0.173 |
| R-free | 0.20290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | THE CRYSTAL STRUCTURE OF MOUSE PP1G IN COMPLEX WITH TRUNCATED HUMAN PPP1R15B INCLUDING RESIDUES OF 631-660 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.053 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | CCP4I |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.940 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.100 | 0.740 |
| Number of reflections | 96347 | |
| <I/σ(I)> | 7.4 | 1.8 |
| Completeness [%] | 92.6 | 99.9 |
| Redundancy | 5.1 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 3 M NACL, 0.1 M HEPES, PH7.5 |
