4V0R

DENGUE VIRUS FULL LENGTH NS5 COMPLEXED WITH GTP AND SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-17
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	94.860, 150.970, 69.280
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.880 - 2.400
R-factor	0.1899
Rwork	0.188
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3P97 AND 2J7U
RMSD bond length	0.006
RMSD bond angle	0.835
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.880
High resolution limit [Å]	2.400
Rmerge	0.120
Number of reflections	39648
<I/σ(I)>	11.2
Completeness [%]	99.8
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		pH 8.5