4V0P
Crystal structure of the MAGE homology domain of human MAGE-A3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-29 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 61.473, 61.473, 292.831 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.394 - 2.070 |
R-factor | 0.202 |
Rwork | 0.200 |
R-free | 0.23810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wa0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.761 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.200 | 2.130 |
High resolution limit [Å] | 2.070 | 2.070 |
Rmerge | 0.060 | 1.110 |
Number of reflections | 21270 | |
<I/σ(I)> | 19.9 | 2.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 10.2 | 10.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1M MG FORMATE, 15% PEG 3350 |