4V0C

Crystal Structure of the Kv7.1 proximal C-terminal Domain in Complex with Calmodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Spacegroup name	P 65
Unit cell lengths	152.092, 152.092, 56.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.784 - 2.860
R-factor	0.228
Rwork	0.226
R-free	0.26790
Structure solution method	MAD
Starting model (for MR)	NONE
RMSD bond length	0.006
RMSD bond angle	1.049
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.080	0.470
Number of reflections	17341
<I/σ(I)>	31.9	6.4
Completeness [%]	99.2	100
Redundancy	15.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.3 M POTASSIUM THIOCYANATE, 0.1 M SODIUM ACETATE TRIHYDRATE PH=5.6, 5 MM CACL2