4V08
Inhibited dimeric pseudorabies virus protease pUL26N at 2 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-30 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.062, 76.226, 111.055 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 62.850 - 2.030 |
R-factor | 0.17346 |
Rwork | 0.170 |
R-free | 0.23432 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v07 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.763 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 62.800 | 2.140 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.110 | 0.780 |
Number of reflections | 29319 | |
<I/σ(I)> | 13.08 | 2.2 |
Completeness [%] | 99.9 | 99.4 |
Redundancy | 6.5 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | PROTEIN SOLUTION WAS INCUBATED WITH 5 MM DFP FOR 1 HOUR, THEN CRYSTALLIZED FROM 0.1 M TRIS/HCL PH 8, 0.2 M MGCL2, 20% PEG 8000 |