4UZQ
STRUCTURE OF THE WNT DEACYLASE NOTUM IN COMPLEX WITH O-PALMITOLEOYL SERINE - CRYSTAL FORM IX - 1.5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00,1.07 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 50.513, 69.350, 120.967 |
| Unit cell angles | 90.00, 92.28, 90.00 |
Refinement procedure
| Resolution | 60.440 - 1.500 |
| R-factor | 0.12888 |
| Rwork | 0.128 |
| R-free | 0.17775 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.882 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 60.000 |
| High resolution limit [Å] | 1.500 |
| Rmerge | 0.100 |
| Number of reflections | 64352 |
| <I/σ(I)> | 11.6 |
| Completeness [%] | 96.4 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 3.0M NACL, 0.1 M CITRATE PH 5.0 |
