4UZA

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM VIII - PHOSPHATE COMPLEX - 2.4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-10
Detector	DECTRIS PILATUS
Spacegroup name	F 4 3 2
Unit cell lengths	232.347, 232.347, 232.347
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	134.150 - 2.400
R-factor	0.21013
Rwork	0.207
R-free	0.27106
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.011
RMSD bond angle	1.537
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	70.000
High resolution limit [Å]	2.400
Rmerge	0.120
Number of reflections	21598
<I/σ(I)>	25
Completeness [%]	100.0
Redundancy	21.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.3		0.630 M K2HPO4 1.170 M NAH2PO4 6.300 PH FINAL PH