4UZ9

STRUCTURE OF THE WNT DEACYLASE NOTUM - CRYSTAL FORM VII - SOS COMPLEX - 2.2A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-15
Detector	DECTRIS PILATUS
Spacegroup name	P 41 21 2
Unit cell lengths	69.630, 69.630, 195.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.580 - 2.200
R-factor	0.19196
Rwork	0.190
R-free	0.23653
Structure solution method	OTHER
Starting model (for MR)	NONE
RMSD bond length	0.014
RMSD bond angle	1.674
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	97.000
High resolution limit [Å]	2.200
Rmerge	0.160
Number of reflections	25372
<I/σ(I)>	13.4
Completeness [%]	100.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		200 MM AMMONIUM SULFATE 100 MM MES 6.5 35 % W/V PEP 629 20MM SOS