4UYI
Crystal structure of the BTB domain of human SLX4 (BTBD12)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-13 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 142.490, 142.490, 50.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 71.245 - 1.860 |
| R-factor | 0.1804 |
| Rwork | 0.180 |
| R-free | 0.19440 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.171 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MR-Rosetta |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.250 | 1.910 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.060 | 0.710 |
| Number of reflections | 16697 | |
| <I/σ(I)> | 24.1 | 3.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.6 | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.2M MAGNESIUM CHLORIDE, 25% PEG3350, 0.1M BIS-TRIS PH 6.5 |
