4UY9
Structure of MLK1 kinase domain with leucine zipper 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-29 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 96.930, 96.930, 416.950 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 77.870 - 2.810 |
R-factor | 0.1889 |
Rwork | 0.187 |
R-free | 0.22800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3dtc |
RMSD bond length | 0.010 |
RMSD bond angle | 1.200 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AMoRE |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.000 | 3.000 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.100 | 0.910 |
Number of reflections | 29688 | |
<I/σ(I)> | 19.2 | 2.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.5 | 13.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |