4UY4
1.86 A structure of human Spindlin-4 protein in complex with histone H3K4me3 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-17 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.072, 51.690, 57.141 |
| Unit cell angles | 81.25, 89.89, 88.00 |
Refinement procedure
| Resolution | 28.683 - 1.862 |
| R-factor | 0.2033 |
| Rwork | 0.202 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4MZF LONGEST CHAIN POLY ALA CONVERTED |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.680 | 5.890 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.060 | 0.360 |
| Number of reflections | 35908 | |
| <I/σ(I)> | 9 | 2.4 |
| Completeness [%] | 97.2 | 91.8 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 33% PEG3350, 0.1M HEPES PH 7.0, SEEDING, 20C |
