4UXR
Conserved mechanisms of microtubule-stimulated ADP release, ATP binding, and force generation in transport kinesins
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK I |
EM Data Collection
Microscope | FEI POLARA 300 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.3 |
Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
Number of Images | 300 |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 7 |
Software | Flex-EM,UCSF Chimera,FREALIGN,SPIDER |