4UWU

Lysozyme soaked with a ruthenium based CORM with a pyridine ligand (complex 7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-18
Detector	ADSC CCD
Spacegroup name	P 43 21 2
Unit cell lengths	78.660, 78.660, 37.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.620 - 1.780
R-factor	0.16282
Rwork	0.161
R-free	0.19522
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.010
RMSD bond angle	1.291
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.620	1.870
High resolution limit [Å]	1.780	1.780
Rmerge	0.050
Number of reflections	11113
Completeness [%]	100.0	100
Redundancy	13.8	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2-10% (M/V) NACL IN 0.1 M ACETATE BUFFER PH 4.5. PROTEIN STOCK CONCENTRATION OF 50 MG/ML. HANGING DROP VAPOR DIFFUSION METHOD, 293K