4UWC

Fibroblast growth factor receptor 1 kinase in complex with JK-P3

Experimental procedure

実験手法	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-19
Detector	ADSC QUANTUM 315
Spacegroup name	C 1 2 1
格子定数 [Å]	207.464, 58.422, 65.946
格子定数 [度]	90.00, 107.38, 90.00

精密化法

分解能	29.210 - 1.960
R因子	0.1789
Rwork	0.177
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	INTERNAL FGFR1 MODEL
結合長の平均二乗偏差(RMSD) [Å]	0.010
結合角の平均二乗偏差(RMSD) [度]	1.030
Data reduction software	XDS (VERSION MAY 10 2010)
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.11.2)

Quality characteristics

	Overall	Outer shell
分解能 [Å] (低)	29.210	2.010
分解能 [Å] (高)	1.960	1.960
Rmerge_l_obs	0.030	0.560
独立反射数	47605
<I/σ(I)>	16.8	1.5
完全性 [%]	87.8	41.1
冗長性	3.4	3.1

結晶化条件

結晶ID	方法	pH	温度	溶液条件
1				20% PEG8000, 20% ETHYLENE GLYCOL, 0.2M AMMONIUM SULPHATE, 0.1M PCTP PH 6.75