4UW6
Human galectin-7 in complex with a galactose based dendron D3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-11 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 58.930, 53.380, 89.220 |
| Unit cell angles | 90.00, 102.89, 90.00 |
Refinement procedure
| Resolution | 45.495 - 1.790 |
| R-factor | 0.2028 |
| Rwork | 0.200 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.437 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.490 | 1.850 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.060 | 0.210 |
| Number of reflections | 24387 | |
| <I/σ(I)> | 8.2 | 3.4 |
| Completeness [%] | 95.2 | 91.5 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.05M BISTRIS PROPANE PH7, 17% PEG 3350 |
