4UW4
Human galectin-7 in complex with a galactose based dendron D2-1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-06-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.950, 58.490, 115.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.500 - 1.766 |
| R-factor | 0.1841 |
| Rwork | 0.182 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bkz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.158 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.500 | 1.830 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.053 | 0.080 |
| Number of reflections | 25707 | |
| <I/σ(I)> | 17 | 8.8 |
| Completeness [%] | 97.1 | 91.7 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.05 M BIS TRIS PROPANE PH 7, 17% PEG 3350 |
