4UW3

Human galectin-7 in complex with a galactose based dendron D1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-23
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	30.410, 65.390, 114.107
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.384 - 1.479
R-factor	0.1767
Rwork	0.176
R-free	0.19770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkz
RMSD bond length	0.007
RMSD bond angle	1.179
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.380	1.560
High resolution limit [Å]	1.480	1.480
Rmerge	0.080	0.490
Number of reflections	38926
<I/σ(I)>	16.5	4.3
Completeness [%]	99.8	98.3
Redundancy	8.4	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.05 M BIS TRIS PROPANE PH7, 17 % PEG 3350