4UVA
LSD1(KDM1A)-CoREST in complex with 1-Methyl-Tranylcypromine (1R,2S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PIXEL |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 118.970, 179.910, 234.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 90.120 - 2.900 |
| R-factor | 0.22036 |
| Rwork | 0.220 |
| R-free | 0.23237 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1d |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.905 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.070 | 0.560 |
| Number of reflections | 55688 | |
| <I/σ(I)> | 13.3 | 2.8 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 4.1 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | PH 6.5 |
