4UUY
Structural Identification of the Vps18 beta-propeller reveals a critical role in the HOPS complex stability and function.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.250, 77.534, 84.508 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 43.310 - 2.140 |
| R-factor | 0.20431 |
| Rwork | 0.201 |
| R-free | 0.24445 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.344 |
| Data reduction software | XDS |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 63.120 |
| High resolution limit [Å] | 2.140 |
| Rmerge | 0.110 |
| Number of reflections | 39106 |
| <I/σ(I)> | 11.3 |
| Completeness [%] | 99.8 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25 % (W/V) PEG 3350, 0.2 M (NH4)2SO4, 0.1 M BIS-TRIS, PH 6.5 |
