4UTN
Crystal structure of zebrafish Sirtuin 5 in complex with succinylated CPS1-peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-14 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 87.440, 87.440, 314.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 75.730 - 3.000 |
R-factor | 0.21782 |
Rwork | 0.215 |
R-free | 0.26672 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nyr |
RMSD bond length | 0.011 |
RMSD bond angle | 1.673 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.180 | 1.340 |
Number of reflections | 15155 | |
<I/σ(I)> | 11.2 | 1.7 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 8.1 | 8.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 20% PEG3350, 0.1 M HEPES PH 7.4 |