4UT6
Crystal structure of dengue 2 virus envelope glycoprotein in complex with the Fab fragment of the broadly neutralizing human antibody EDE2 B7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-07 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 101.277, 58.980, 191.573 |
Unit cell angles | 90.00, 96.20, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.200 |
R-factor | 0.214 |
Rwork | 0.212 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1OKE 3KDM AND 3H0T |
RMSD bond length | 0.008 |
RMSD bond angle | 1.130 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.340 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.110 | 0.640 |
Number of reflections | 37500 | |
<I/σ(I)> | 9.2 | 1.6 |
Completeness [%] | 99.3 | 99 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 100MM TRIS PH 8.5, 16% (W/V) PEG 400, 200MM MGCL2, CRYOPROTECTED IN 22%(V/V) GLYCEROL |