4URZ

The crystal structure of H-Ras and SOS in complex with ligands

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-27
Detector	ADSC QUANTUM 315r
Spacegroup name	I 4 2 2
Unit cell lengths	150.206, 150.206, 201.622
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.230 - 2.240
R-factor	0.1917
Rwork	0.191
R-free	0.21240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bkd
RMSD bond length	0.010
RMSD bond angle	1.030
Data reduction software	MOSFLM
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.980	2.490
High resolution limit [Å]	2.360	2.360
Rmerge	0.070	0.490
Number of reflections	46823
<I/σ(I)>	17.1	3.6
Completeness [%]	100.0	100
Redundancy	7.2	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1