4URM
Crystal Structure of Staph GyraseB 24kDa in complex with Kibdelomycin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2009-11-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.280, 75.550, 90.450 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 30.440 - 2.940 |
| R-factor | 0.1984 |
| Rwork | 0.195 |
| R-free | 0.25630 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 90.450 | 90.450 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Rmerge | 0.090 | |
| Number of reflections | 20139 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.12M MAGNESIUM CHLORIDE, 0.06 M TRIS PH8.5, 15% PEG 3350 |
