4UJ5
Crystal structure of human Rab11-Rabin8-FIP3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Detector | DECTRIS PILATUS |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.683, 108.202, 75.736 |
Unit cell angles | 90.00, 102.83, 90.00 |
Refinement procedure
Resolution | 43.642 - 2.604 |
R-factor | 0.2174 |
Rwork | 0.215 |
R-free | 0.27160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yzk |
RMSD bond length | 0.005 |
RMSD bond angle | 1.072 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.530 | 2.530 |
Rmerge | 0.040 | 0.740 |
Number of reflections | 24951 | |
<I/σ(I)> | 14 | 1 |
Completeness [%] | 94.0 | 84 |
Redundancy | 3.5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7 | 0.1M HEPES PH 7.0, 0.2M LITHIUM SULFATE AND 24% PEG 3350 |