4UJ3
Crystal structure of human Rab11-Rabin8-FIP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M-F |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.498, 165.374, 218.687 |
| Unit cell angles | 90.00, 95.93, 90.00 |
Refinement procedure
| Resolution | 48.543 - 3.000 |
| R-factor | 0.2005 |
| Rwork | 0.198 |
| R-free | 0.25040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yzk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.990 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1647)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.140 |
| High resolution limit [Å] | 3.000 | 2.970 |
| Rmerge | 0.050 | 0.650 |
| Number of reflections | 92398 | |
| <I/σ(I)> | 19 | 1.8 |
| Completeness [%] | 95.0 | 94 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 50MM MES PH 5.6, 0.2M AMMONIUM SULFATE AND 12% PEG 8000 |
