4UHZ
Crystal structure of the human RGMB-BMP2 complex, crystal form 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-07 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 129.010, 129.010, 129.010 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.850 |
| R-factor | 0.206 |
| Rwork | 0.205 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.050 | 0.820 |
| Number of reflections | 8429 | |
| <I/σ(I)> | 15.1 | 1.5 |
| Completeness [%] | 99.4 | 98.9 |
| Redundancy | 4.2 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M AMMONIUM SULPHATE, 8% (V/V) 2,5- HEXANEDIOL |
