4UFR
Structure of the ectodomain of LGR5 in complex with R-spondin-2 (Fu1Fu2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-25 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 205.148, 59.777, 112.210 |
| Unit cell angles | 90.00, 99.51, 90.00 |
Refinement procedure
| Resolution | 110.670 - 2.200 |
| R-factor | 0.20909 |
| Rwork | 0.208 |
| R-free | 0.26172 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.635 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 69.000 |
| High resolution limit [Å] | 2.200 |
| Rmerge | 0.120 |
| Number of reflections | 62167 |
| <I/σ(I)> | 10.8 |
| Completeness [%] | 90.6 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 25 %W/V PEG3350, 0.200 M LITHIUM SULPHATE, 0.100 M BIS-TRIS PH 5.5 |
