4UED

Complex of human eIF4E with the 4E binding protein 4E-BP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-25
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 1 21 1
Unit cell lengths	42.110, 66.580, 43.050
Unit cell angles	90.00, 118.30, 90.00

Refinement procedure

Resolution	37.906 - 1.750
R-factor	0.1946
Rwork	0.192
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4TPW CHAIN A
RMSD bond length	0.004
RMSD bond angle	0.780
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((PHENIX.REFINE: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.900	1.800
High resolution limit [Å]	1.750	1.750
Number of reflections	20595
<I/σ(I)>	13.4	2.5
Completeness [%]	96.9	98.1
Redundancy	2.9	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		0.03M EACH DIETHYLENE GLYCOL, TRIETHYLENE GLYCOL, TETRAETHYLENE GLYCOL, PENTAETHYLENE GLYCOL, 0.1M BICINE/TRIZMA BASE PH 8.5, 12.5% PEG1000, 12.5% PEG3350, 12.5% MPD