4UE0
Structure of the bovine atadenovirus type 4 fibre head protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-21 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 |
Unit cell lengths | 43.330, 48.190, 52.070 |
Unit cell angles | 117.11, 95.56, 110.17 |
Refinement procedure
Resolution | 44.150 - 1.170 |
R-factor | 0.11699 |
Rwork | 0.116 |
R-free | 0.14595 |
Structure solution method | SIRAS |
Starting model (for MR) | NONE |
RMSD bond length | 0.012 |
RMSD bond angle | 1.549 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.150 | 1.210 |
High resolution limit [Å] | 1.170 | 1.170 |
Rmerge | 0.030 | 0.350 |
Number of reflections | 103174 | |
<I/σ(I)> | 15.7 | 2.6 |
Completeness [%] | 91.2 | 86.7 |
Redundancy | 3.6 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% (W/V) PEG3350, 200 MM KSCN, 50 MM NACL, 10 MM TRIS-HCL PH 7.5, 5% (V/V) GLYCEROL | |||
1 | 20% (W/V) PEG3350, 200 MM KSCN, 50 MM NACL, 10 MM TRIS-HCL PH 7.5, 5% (V/V) GLYCEROL |