4UCB
N-terminal globular domain of the RSV Nucleoprotein in complex with C- terminal peptide of the Phosphoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.520, 71.680, 178.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.790 |
| R-factor | 0.1979 |
| Rwork | 0.195 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wj8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.930 |
| High resolution limit [Å] | 2.780 | 2.780 |
| Rmerge | 0.120 | 0.420 |
| Number of reflections | 45949 | |
| <I/σ(I)> | 8.4 | 1.7 |
| Completeness [%] | 94.7 | 71.1 |
| Redundancy | 4.1 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
