4UCA
N-terminal globular domain of the RSV Nucleoprotein in complex with C- terminal peptide of the Phosphoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.840, 71.580, 177.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.220 |
| R-factor | 0.1955 |
| Rwork | 0.192 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wj8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 3.400 |
| High resolution limit [Å] | 3.220 | 3.220 |
| Rmerge | 0.180 | 0.510 |
| Number of reflections | 7219 | |
| <I/σ(I)> | 7.7 | 1.7 |
| Completeness [%] | 96.9 | 86 |
| Redundancy | 4.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
