4UC5
Neisseria Meningitidis DAH7PS-Phenylalanine regulated
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2013-11-26 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.607, 143.460, 75.179 |
| Unit cell angles | 90.00, 96.18, 90.00 |
Refinement procedure
| Resolution | 74.740 - 2.190 |
| R-factor | 0.19141 |
| Rwork | 0.189 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hsn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.271 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER (MR) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.820 | 2.230 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.080 | 1.050 |
| Number of reflections | 79522 | |
| <I/σ(I)> | 9.3 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.3 | 0.1 M TRIS HCL (PH 7.3), 0.2 M TRIMETHYL-AMINO-N-OXIDE (TMAO), 600UM MNSO4 AND 15-20% (W/V) PEG 2000MME |
