4UC1
High resolution crystal structure of translocator protein 18kDa (TSPO) from Rhodobacter sphaeroides (A139T Mutant) in C2 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 58.632, 99.544, 95.210 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 43.965 - 1.800 |
| R-factor | 0.2023 |
| Rwork | 0.201 |
| R-free | 0.24670 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.155 |
| Data scaling software | XDS |
| Phasing software | SHARP (2.8.2) |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.970 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.087 | |
| Number of reflections | 97994 | |
| <I/σ(I)> | 10.6 | 1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7.5 | 293 | 32% PEG 400, 0.05 M NaCl, 0.04 M MgCl2 and 0.1 M HEPES buffer |
