4UBF
HsMCAK motor domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-07-11 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.306, 245.644, 79.399 |
| Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
| Resolution | 29.492 - 3.000 |
| R-factor | 0.2652 |
| Rwork | 0.264 |
| R-free | 0.28630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2heh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.227 |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.091 | 0.682 |
| Number of reflections | 35146 | |
| <I/σ(I)> | 10.2 | 2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.4 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 292 | 24 % w/v PEG 1500 20 % v/v Glycerol Protein crystals appeared after two days and single crystals were used for measurement |
