4UAO

Crystal structure of Apical Membrane Antigen 1 from Plasmodium Knowlesi in complex with an invasion inhibitory antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	165.864, 71.807, 90.688
Unit cell angles	90.00, 116.37, 90.00

Refinement procedure

Resolution	40.770 - 3.100
R-factor	0.1925
Rwork	0.188
R-free	0.26620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1w81 2gcy 1d5i 1igf
RMSD bond length	0.010
RMSD bond angle	1.310
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.350	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.152	0.778
Number of reflections	17127
<I/σ(I)>	7.8	1.3
Completeness [%]	97.8	99.1
Redundancy	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	290	8% PEG 6000, 40 mM Hepes pH7.4, 80 mM NaCl