4UAF

Importin alpha 1 delta IBB in complex with Influenza PB2 nuclear localization domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	77.131, 90.188, 100.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.963 - 1.698
R-factor	0.1654
Rwork	0.165
R-free	0.19270
RMSD bond length	0.007
RMSD bond angle	1.060
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	1.700
Number of reflections	76101
<I/σ(I)>	48.5
Completeness [%]	97.5
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.49 M Na phosphate monobasic 0.91 M K phosphate dibasic pH 6.9