4U8U
The Crystallographic structure of the giant hemoglobin from Glossoscolex paulistus at 3.2 A resolution.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X26C |
Synchrotron site | NSLS |
Beamline | X26C |
Temperature [K] | 103 |
Detector technology | CCD |
Collection date | 2009-07-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 272.680, 319.900, 333.180 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.655 - 3.200 |
R-factor | 0.2165 |
Rwork | 0.215 |
R-free | 0.23520 |
RMSD bond length | 0.012 |
RMSD bond angle | 0.962 |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.112 | 0.500 |
Number of reflections | 237062 | |
<I/σ(I)> | 11.2 | 3.1 |
Completeness [%] | 99.8 | 99.2 |
Redundancy | 4.7 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | PEG8000 10%, CaCl2 2.5 mM, TRIS 50 mM |